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  • Frontiers in Optics 2004/Laser Science XXII/Diffractive Optics and Micro-Optics/Optical Fabrication and Testing
  • OSA Technical Digest Series (Optica Publishing Group, 2004),
  • paper FTuE2
  • https://doi.org/10.1364/FIO.2004.FTuE2

Protein sensing with SERS: insulin variants and phosphorylation detection

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Abstract

Spectral differences of surface enhanced Raman scattering (SERS) were observed for human insulin versus analog and for phosphorylated protein versus unphosphorylated. Adaptive silver films enable soft protein deposition and improved SERS enhancement. This allows to detect small structural differences of proteins at sub-monolayer density.

© 2004 Optical Society of America

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