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Fast Cardiac Imaging in Live Embryos Using Multiphoton Light-sheet Microscopy at Low Laser Repetition Rate

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, and Willy Supatto

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Abstract

We propose a framework to quantify photodamage in multiphoton light-sheet microscopy. Using cardiac imaging in live zebrafish embryos, we demonstrate an order of magnitude signal enhancement is safely obtained by adjusting the laser repetition rate.

© 2021 The Author(s)

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