Adaptive Optics Light-Sheet Microscopy for Functional Neuroimaging

Antoine Hubert; Antoine Hubert; Fabrice Harms; Sophia Imperato; Vincent Loriette; Cynthia Veilly; Xavier Levecq; Georges Farkouh; François Rouyer; Alexandra Fragola

doi:10.1364/ECBO.2021.EM2B.1

European Conferences on Biomedical Optics 2021 (ECBO)
OSA Technical Digest (Optica Publishing Group, 2021),
paper EM2B.1
•https://doi.org/10.1364/ECBO.2021.EM2B.1

Adaptive Optics Light-Sheet Microscopy for Functional Neuroimaging

Antoine Hubert, Fabrice Harms, Sophia Imperato, Vincent Loriette, Cynthia Veilly, Xavier Levecq, Georges Farkouh, François Rouyer, and Alexandra Fragola

European Conference on Biomedical Optics 2021

Munich Germany
20–24 June 2021
ISBN: 978-1-943580-95-8

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Light-sheet and Volumetric Imaging Techniques (EM2B)

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A. Hubert, F. Harms, S. Imperato, V. Loriette, C. Veilly, X. Levecq, G. Farkouh, F. Rouyer, and A. Fragola, "Adaptive Optics Light-Sheet Microscopy for Functional Neuroimaging," in European Conferences on Biomedical Optics 2021 (ECBO), OSA Technical Digest (Optica Publishing Group, 2021), paper EM2B.1.

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Abstract

We present a new implementation of adaptive optics for light-sheet microscopy, with a direct extended-scene wavefront sensing measurement for fast aberration correction. We report AO-enhanced images of GCaMP in freshly dissected drosophila brains.

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