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  • Optical Coherence Imaging Techniques and Imaging in Scattering Media III
  • SPIE Proceedings (Optica Publishing Group, 2019),
  • paper 11078_66
  • https://doi.org/10.1117/12.2527163

Durable 3D test standards from mouse retina for performance testing of high-resolution optical coherence tomography systems

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Abstract

For performance testing of high-resolution label-free optical coherence tomography (OCT) systems, durable test stand-ards with optical properties similar to native biological tissues are highly desirable. Therefore, we explored the estab-lishment of ex-vivo 3D samples from dissected mouse retina. To achieve high stability, dissected tissue parts were em-bedded in EPON resin. Results from comparative investigations with OCT on murine retina in agarose demonstrate that by utilization of appropriate EPON embedding protocols durable samples with adequate refractive index properties can be created in which even tiny structures near the OCT resolution limits can be detected.

© 2019 SPIE/OSA

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