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Conferences > ECBO > 2019 > PSW11076 > 11076_79

Two-photon excited polarized fluorescence in NADH in methanol/water solutions

I. A. Gorbunova, N. O. Bezverkhnii, A. A. Zhikhoreva, Y. M. Beltukov, M. E. Sasin, J. Rubayo-Soneira, and O. S. Vasyutinskii

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Abstract

We studied polarized fluorescence in biological coenzyme NADH in water-methanol solutions upon two-photon excitation with femtosecond laser pulses at 720 nm. The polarized fluorescence decay was recorded by a time correlated single photon counting (TCSPC) system. Fluorescence decay times, rotational diffusion time, fluorescence anisotropy, and the ratio of two pre-exponential factors have been determined and studied as a function of methanol concentration. The results obtained were interpreted on the basis of a model of NADH denaturation processes in solutions and can be used for modeling of NADH binding with various dehydrogenases in living cells.

© 2019 SPIE/OSA

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