One of the drawbacks of using Lloyd’s mirror interferometer for quantitative phase contrast microscopy is that it leads to overlapping of the object information which restricts the use of field of view. One way to overcome this limitation is to prepare the sample slide in such a way that the object information is contained in only one portion of the illuminating beam, while the other acts as a separate reference beam. This geometry has excellent temporal stability and is used to examine human erythrocytes.

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