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Nonlinear imaging of female breast tissue biopsies

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Abstract

In this study nonlinear microscopy techniques utilized to shed new light in breast cancer diagnosis. In particular, the nonlinear imaging modalities of multi-photon excitation fluorescence (MPEF) second and third harmonic generation (SHG, THG) used as non-destructive, label free diagnostic tools for the qualitative discrimination of breast cancer tissues. Nonlinear signals were collected from unstained thin histological sections of breast biopsies samples. In an attempt to discriminate the different types of tissues, quantitative analysis of the recorded THG signals on specific cells in tissue was also performed.

© 2019 SPIE/OSA

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