Abstract
Flow cytometry is the main technology used in hematology analyzers. However, this technology requires bulky and complex hardware systems. Lens-free imaging is an emerging microscopy technique based on a simple and compact in-line holography setup. This technique enables to image a large field-of-view (≈30mm2) leading to statistical counting (>10 000 cells) in a single-shot acquisition consistent with performances required in hematology. We report high accuracy platelet count in 54 platelet-rich plasma samples. This accuracy can be achieved through a wise choice of the illumination spectral properties and an optimized algorithmic chain dedicated to small pure phase objects.
© 2019 SPIE/OSA
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