Abstract

We report on the deep-tissue imaging using novel upconversion nanoparticles (UCNPs) β-NaYF4:Yb3+,Tm3+ (excitation wavelength: 975 nm, detection wavelength: 800 nm) and glycerol as an optical clearing agent to enhance imaging from under 6-mm-thick porcine muscle tissue samples. We show that improvement of luminescent label visualization is caused by transforming of the diffuse label-emitted light into the direct component. This results in the increase in visibility (ratio of the sum and difference of the maximal and minimal intensity) of the label and increase in maximal signal intensity thus making the combination of the phosphors and optical clearing promising for precise detection of tissue-embedded labelled inhomogeneities.

© 2015 SPIE

PDF Article
More Like This
High-resolution deep-tissue optical imaging using anti-Stokes phosphors

A.P. Popov, A.V. Karmenyan, A.V. Bykov, E.V. Khaydukov, A.V. Nechaev, O.A. Bibikova, V.Ya. Panchenko, V.A. Semchishen, V.N. Seminogov, A.S. Akhmanov, V.I. Sokolov, M.T. Kinnunen, V.V. Tuchin, and A.V. Zvyagin
88010C European Conference on Biomedical Optics (ECBO) 2013

Controlling of upconversion nanoparticle luminescence at heating and optical clearing of adipose tissue

Irina Yu. Yanina, Elena K. Volkova, Daria K. Tuchina, Julia G. Konyukhova, Vyacheslav I. Kochubey, and Valery V. Tuchin
1041709 European Conference on Biomedical Optics (ECBO) 2017

Upconversion luminophores as a novel tool for deep tissue imaging

Alexey P. Popov, Alexander V. Bykov, Victor I. Sokolov, Yulia V. Lysak, Annemarie Nadort, Alexander V. Priezzhev, Risto Myllylä, and Andrei V. Zvyagin
80900V European Conference on Biomedical Optics (ECBO) 2011

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription