3D Imaging of apoptosis by FRET, light sheet fluorescence and scattering microscopy

Herbert Schneckenburger; Petra Weber; Sarah Schickinger; Verena Richter; Thomas Bruns; Michael Wagner

doi:10.1364/ECBO.2015.953716

Clinical and Biomedical Spectroscopy and Imaging IV
SPIE Proceedings (Optica Publishing Group, 2015),
paper 953716
•https://doi.org/10.1364/ECBO.2015.953716

3D Imaging of apoptosis by FRET, light sheet fluorescence and scattering microscopy

Herbert Schneckenburger, Petra Weber, Sarah Schickinger, Verena Richter, Thomas Bruns, and Michael Wagner

European Conference on Biomedical Optics 2015

Munich Germany
21–25 June 2015
ISBN: 9781628417029

From the session
NOVEL TECHNIQUES IN DIAGNOSIS, THERAPY, AND MONITORING (NTDTM)

Not Accessible

Your library or personal account may give you access

Get PDF
Email
Share
Get Citation
Copy Citation Text
H. Schneckenburger, P. Weber, S. Schickinger, V. Richter, T. Bruns, and M. Wagner, "3D Imaging of apoptosis by FRET, light sheet fluorescence and scattering microscopy," in Clinical and Biomedical Spectroscopy and Imaging IV, J. Brown and V. Deckert, eds., Vol. 9537 of SPIE Proceedings (Optica Publishing Group, 2015), paper 953716.

Export Citation
- BibTex
- Endnote (RIS)
- HTML
- Plain Text
Citation alert
Save article

Abstract

Non-radiative cell membrane associated energy transfer (FRET) from a cyan (ECFP) to a yellow (EYFP) fluorescent protein is used for detection of apoptosis in multi-cellular tumor spheroids. Low light exposure in light sheet fluorescence microscopy is combined with the detection of spectral changes and prolonged fluorescence lifetimes of the donor ECFP upon apoptosis. In view of future label-free detection preliminary experiments of light scattering microscopy with high angular resolution are reported which may give additional information on morphological changes.

PDF Article

More Like This

A Membrane-Associated FRET Sensor for Detection of Apoptosis

Herbert Schneckenburger, Michael Wagner, Petra Weber, Thomas Bruns, Heiko Steuer, and Brigitte Angres
7368_0Q European Conference on Biomedical Optics (ECBO) 2009

High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)

Stylianos E. Psycharakis, Evangelos Liapis, Athanasios Zacharopoulos, Mariam-Eleni Oraiopoulou, Chrysoula Aivalioti, Vangelis Sakkalis, Joseph Papamatheakis, Jorge Ripoll, and Giannis Zacharakis
11076_24 European Conference on Biomedical Optics (ECBO) 2019

Analysis of the 3D structure of agglutinated erythrocyte using CellScan and Confocal microscopy. Characterization by FLIM-FRET.

B. Riquelme, D. Dumas, J. Valverde, R. Rasia, and J. F. Stoltz
5139_190 European Conference on Biomedical Optics (ECBO) 2003

Previous Article Next Article