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Conferences > ECBO > 2013 > CPDI > 879804
  • Clinical and Biomedical Spectroscopy and Imaging III
  • SPIE Proceedings (Optica Publishing Group, 2013),
  • paper 879804

Visualizing a Cytostatic Drug and Probing Apoptosis of Cancer Cells

Verena Richter, Petra Weber, Michael Wagner, Sarah Schickinger, Thomas Bruns, and Herbert Schneckenburger

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Abstract

The uptake and intracellular distribution of the cytostatic drug doxorubicin is visualized in 2D and 3D systems of human breast cancer cells and fibroblasts by fluorescence microscopy and spectroscopy. Fluorescence lifetime imaging (FLIM) and scattering experiments with high angular resolution are suggested to probe apoptotic reactions. A light scattering microscope as well as a light sheet module for 3D fluorescence microscopy have been developed and are used for this purpose.

© 2013 SPIE

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