The uptake and intracellular distribution of the cytostatic drug doxorubicin is visualized in 2D and 3D systems of human breast cancer cells and fibroblasts by fluorescence microscopy and spectroscopy. Fluorescence lifetime imaging (FLIM) and scattering experiments with high angular resolution are suggested to probe apoptotic reactions. A light scattering microscope as well as a light sheet module for 3D fluorescence microscopy have been developed and are used for this purpose.

© 2013 SPIE

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