Oligonucleotide Switches and Nanomaterials for Intracellular mRNA Sensing

S. Tombelli; M. Ballestri; G. Giambastiani; A. Giannetti; A. Guerrini; G. Sotgiu; C. Trono; G. Tuci; G. Varchi; F. Baldini

Clinical and Biomedical Spectroscopy and Imaging III
SPIE Proceedings (Optica Publishing Group, 2013),
paper 879802

Oligonucleotide Switches and Nanomaterials for Intracellular mRNA Sensing

S. Tombelli, M. Ballestri, G. Giambastiani, A. Giannetti, A. Guerrini, G. Sotgiu, C. Trono, G. Tuci, G. Varchi, and F. Baldini

European Conference on Biomedical Optics 2013

Munich Germany
12–16 May 2013
ISBN: 9780819496478

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Biospectroscopy and Poc Diagnostics (BPD)

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S. Tombelli, M. Ballestri, G. Giambastiani, A. Giannetti, A. Guerrini, G. Sotgiu, C. Trono, G. Tuci, G. Varchi, and F. Baldini, "Oligonucleotide Switches and Nanomaterials for Intracellular mRNA Sensing," in Clinical and Biomedical Spectroscopy and Imaging III, V. Deckert and N. Ramanujam, eds., Vol. 8798 of SPIE Proceedings (Optica Publishing Group, 2013), paper 879802.

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Abstract

We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the nanoparticles.

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