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Conferences > ECBO > 2013 > PSI > 87970L
  • Advanced Microscopy Techniques III
  • SPIE Proceedings (Optica Publishing Group, 2013),
  • paper 87970L

Multispot two-photon imaging of calcium waves dynamics in cardial tissue at 16Hz frame rate

C. de Mauro, C. A. Cecchetti, D. Alfieri, G. Borile, A. Urbani, M. Mongillo, and F. S. Pavone

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Abstract

We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dynamics at intracellular level. Time resolution of a few milliseconds, even in full frame images at 512×512 pixels, is achieved, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue.

© 2013 SPIE

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