Abstract

In digital holographic microscopy (DHM), scattering patterns that are induced by coherent laser light affect the resolution for the detection of optical path length changes. We present a simple and efficient approach for the reduction of coherent disturbances in quantitative phase imaging in self-interference DHM that is based on amplitude and phase modulation of the sample illumination. The performance of the method for quantitative phase imaging is characterized and the application for quantitative analysis of living cells is illustrated.

© 2013 SPIE

PDF Article
More Like This
Digital Filtering Approaches for Noise Reduction in Quantitative Digital Holographic Phase Contrast Imaging of Living Cells

Lena Tacke, Klaus Brinker, and Björn Kemper
Th3A.38 Digital Holography and Three-Dimensional Imaging (DH) 2019

Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy

Björn Kemper, Luisa Pohl, Mathias Kaiser, Eva Döpker, Jürgen Schnekenburger, and Steffi Ketelhut
11076_30 European Conference on Biomedical Optics (ECBO) 2019

Quantitative phase imaging with digital holographic microscopy applied for label-free analysis of confluent cell layers

Björn Kemper, Luisa Pohl, Mathias Kaiser, Jürgen Schnekenburger, and Steffi Ketelhut
Th1A.4 Digital Holography and Three-Dimensional Imaging (DH) 2019

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription