Imaging of cells is an interesting and challenging problem as they do not appreciably change the amplitude of the electromagnetic radiation interacting with them. Phase contrast techniques can be used to overcome this hurdle. Interferometric phase contrast techniques like digital holography can be used for quantitative phase contrast microscopic imaging of transparent objects and it yields the three dimensional profile of the object under investigation. These methods also have advantage of numerical focusing, allowing one to focus on to any desired object plane. But most of the interferometric quantitative phase contrast techniques require two beams as well as the adjustment of the beams for high fringe contrast, requiring stringent optical conditions. Here we present a single beam phase retrieval technique for quantitative phase contrast microscopy of cells. The phase information of the object is obtained by sampling the volume speckle field generated by the object at several axial planes. These intensity patterns are used iteratively in the diffraction integral to retrieve the phase information about the object. The advantages of this technique include compactness, immunity to external vibrations as well as the prospect of usage of low coherent sources.

© 2011 OSA/SPIE

PDF Article
More Like This
Dynamic Differential Image Contrast by Digital Holography for imaging and Quantitative Phase Microscopy

L. Miccio, A. Finizio, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro
80860D European Conference on Biomedical Optics (ECBO) 2011

Quantitative live cell imaging with tomographic phase microscopy

Wonshik Choi, Yongjin Sung, Yongkeun Park, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld
NTuA1 Novel Techniques in Microscopy (NTM) 2009

Wide field of view self-referencing quantitative phase contrast microscopy based on Lloyd’s mirror interferometer

Mugdha Joglekar, Vismay Trivedi, Swapnil Mahajan, Vani Chhaniwal, Rainer Leitgeb, Giancarlo Pedrini, Bahram Javidi, and Arun Anand
11076_29 European Conference on Biomedical Optics (ECBO) 2019


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access OSA Member Subscription