Abstract
A method to quantify fluorescent labels spatially resolved in scattering and absorbing samples is proposed and tested using a tissue phantom. The method works without any a priori knowledge about the optical properties of the sample. The scattering and absorption behavior of the sample is estimated by measuring reflectance from the sample simultaneously to the fluorescence. With this estimation, the attenuation of the fluorescence caused by scattering and absorption can be mathematically compensated. The method is planned to be used for evaluating skin penetrating drug carrier systems.
© 2011 OSA/SPIE
PDF ArticleMore Like This
Ulf Maeder, Thorsten Bergmann, Jan Michael Burg, Sebastian Beer, Peggy Schlupp, Thomas Schmidts, Johannes T. Heverhagen, Frank Runkel, and Martin Fiebich
80861A European Conference on Biomedical Optics (ECBO) 2011
Amin Feizpour, Louise Bastholm, Troels T. Marstrand, and Conor L. Evans
JW3C.1 Bio-Optics: Design and Application (BODA) 2019
Goro Nishimura, Kamlesh Awasthi, Kitsakorn Locharoenrat, Shinpei Okawa, and Yukio Yamada
BTuD11 Biomedical Optics (BIOMED) 2010