Photodynamic inactivation (PDI) of bacterial strains presents an attractive potential alternative to antibiotic therapies. Success is dependent on the effective accumulation in bacterial cells of photochemical substances called photosensitizers, which are usually porphyrins or their derivatives. The kinetics of porphyrin synthesis after treatment with the precursor ALA and the accumulation of the Chlorin e6 and the following illumination were studied. The goal was to estimate effectivity of the destructive power of these PS in vitro in respect of the physiological states of Mycobacteria. So the present results examine the cell destruction by PDI using ALA-induced Porphyrins and Chlorin e6 accumulated in Mycobacterium phlei and Mycobacterium smegmatis, which serve as models for the important pathogens Mycobacterium tuberculosis, Mycobacterium leprae and Mycobacterium bovis. We could show that both Mycobacterium after ALA and Chlorin e6 application were killed by illumination with light of about 662 nm. A reduction of about 97 % could be reached by using a light-dose of 70 mW/cm2.

© 2009 OSA/SPIE

PDF Article
More Like This
The Susceptibility of Bacteria to Photodynamic Inactivation with Lanthanides Conjugates of Fotolon (chlorin e6)

Z. Drulis-Kawa, A. Bednarkiewicz, W. Stręk, J. Legendziewicz, and R. Wiglusz
TuA4 European Conference on Biomedical Optics (ECBO) 2005

Merocyanine-540 Mediated Photodynamic Effects on Staphylococcus epidermidis Biofilms

Maria Sonia Sbarra, Antonella Di Poto, Enrica Saino, Livia Visai, Paolo Minzioni, Francesca Bragheri, and Ilaria Cristiani
7373_0R European Conference on Biomedical Optics (ECBO) 2009

In vitro Study for Photodynamic Therapy using Fotolon® in Glioma Treatment

Sara Abdel Hamid, Wolfgang Zimmermann, Dirk Huettenberger, Rainer Wittig, Mahmoud Abdel Kader, and Herbert Stepp
95420B European Conference on Biomedical Optics (ECBO) 2015


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access OSA Member Subscription