Abstract

Depth resolved absorption profiles in the wavelength range of 800nm and 140nm bandwidth are demonstrated using spectroscopic frequency domain OCT. Absorption dynamics are presented, which might be useful for the investigation of pharmacokinetics or pharmacodynamics.

In this study, depth resolved measurements of absorption profiles in the wavelength range of 800 nm with a bandwidth of 140 nm are demonstrated using high speed spectroscopic frequency domain OCT (SOCT). With proper calibration, SOCT is able to extract absolute, depth resolved absorption profiles over the whole wavelength range at once without the need of tuning and performing measurements at single wavelengths sequentially. In addition, high acquisition speed in excess of 20 kHz allows to measure absorption dynamics with 50 μs time resolution, which might be useful for the investigation of pharma-cokinetics or pharmacodynamics.

SOCT of ~600 μm thick single- and multilayered, weakly scattering phantoms with varying absorption in the range of 10–60 cm−1, equivalent to blood absorption in capillaries, is presented. SOCT measurements are compared with those using a spectrometer in transmission mode. For Indocyanine Green (ICG), a dynamic absorption measurement is demonstrated.

© 2009 OSA/SPIE