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Conferences > ECBO > 2009 > TLCI > 7370_09

Improving Precision in Time-Gated FLIM for Low-Light Live-Cell Imaging

Ching-Wei Chang and Mary-Ann Mycek

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Abstract

Minimizing stress to live-cell systems during imaging is critical. Time-gating optimization and image denoising were employed independently and in combination to significantly improve precision in low-light time-gated FLIM.

© 2009 OSA/SPIE

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