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Using Pd-porphyrin phosphorescence and photodynamic oxygen consumption to study oxygen diffusion in cells

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Abstract

MLL cells were incubated with Pd-porphyrin and irradiated at 405 nm. The change in Pd-porphyrin phosphorescence intensity was monitored during treatment. A time dependent diffusion model has been developed to fit the measured phosphorescence signals so that the value of the oxygen diffusion coefficient in cells can be calculated

© 2009 OSA/SPIE

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