Optical second harmonic generation, thanks to its coherent nature, is a suitable signal for interferometric measurements, such as digital holography: a well-established imaging technique that allows recovering of the complex diffraction wavefields from which it is possible to extract both amplitude-contrast and quantitative phase images. We believe that application of digital holography to non-linear optical fields might ultimately be the key to phase-related functional imaging. In a first approach, we report here on second harmonic generation digital holographic microscopy, and present its application to (1) discrimination of nanoparticles of different nature — here polystyrene microspheres and barium titanate (BaT iO3) nanoparticles — and to (2) 3D-mapping of a distribution of BaT iO3 nanoparticles.

© 2009 OSA/SPIE

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