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Conferences > ECBO > 2007 > IMII > 6630_24

Aberration-free refocusing in high numerical aperture microscopy

Tony Wilson, Edward Botcherby, Rimas Juškaitis, and Martin Booth

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Abstract

We describe a method of optical refocusing for high numerical aperture systems that is particularly relevant for confocal and multiphoton microscopy. Crucially the method avoids spherical aberration that is common to other optical refocussing systems. Further refocusing is implemented remotely from the specimen.

© 2007 SPIE

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