Development of a TIRF-FLIM microscope for biomedical applications

P. Blandin; P. Blandin; S. Lévêque-Fort; S. Lécart; P. Zeller; Z. Lenkei; F. Druon; P. Georges

doi:10.1364/ECBO.2007.6630_10

Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2007),
paper 6630_10
•https://doi.org/10.1364/ECBO.2007.6630_10

Development of a TIRF-FLIM microscope for biomedical applications

P. Blandin, S. Lévêque-Fort, S. Lécart, P. Zeller, Z. Lenkei, F. Druon, and P. Georges

European Conference on Biomedical Optics 2007

Munich Germany
17–21 June 2007
ISBN: 9780819467744

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FLUORESCENCE LIFETIME IMAGING MICROSCOPY (FLM)

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P. Blandin, S. Lévêque-Fort, S. Lécart, P. Zeller, Z. Lenkei, F. Druon, and P. Georges, "Development of a TIRF-FLIM microscope for biomedical applications," in Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, A. Periasamy, ed., Vol. 6630 of Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2007), paper 6630_10.

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Abstract

Total internal reflection fluorescence microscopy (TIRFM) is a powerful optical technique to observe fluorescence close to surfaces. Associated with fluorescence lifetime imaging, TIRFM enables to measure contrasts independent of fluorophores concentration and reveal intracellular activity with subwavelength axial resolution. We developed an original setup which allows, thanks to a wide-field time resolved detection, to measure nanoseconds fluorescence lifetimes of membrane receptors in order to apprehend their signalisation pathway.

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