Abstract

Light-induced autofluorescence spectroscopy provides many possibilities for medical diagnostics needs for differentiation of tissue pathologies including cancer. For the needs of clinical practice scientists collect spectral data from patients in vivo or they study different tumor models to obtain objective information for fluorescent properties of every kind of normal and diseased tissue. Therefore it is very important to find the most appropriate and close to the human skin samples from the point of view of laser-induced fluorescence spectroscopy, which will give the possibility for easier transfer of data obtained in animal models to spectroscopic medical diagnostics in humans. In this study are presented some results for in vitro detection of the autofluorescence signals of the animal skin (pig and chicken) with using of LEDs as excitation sources (maximum emission at 365, 375, 385 and 400 nm). The autofluorescence signals from in vivo human skin were also detected for comparison with the models’ results. Specific features of the spectra measured are discussed and there are proposed some of the origins of the fluorescence signals obtained. Fluorescence maxima detected are addressed to the typical fluorophores existing in the cutaneous tissues. Influence of main skin absorbers, namely melanin and hemoglobin, is also discussed.

© 2007 SPIE

PDF Article
More Like This
A diffusion approximation model of light transport in multilayered skin tissue

M. Makropoulou, E. Kaselouris, E. Drakaki, A.A. Serafetinides, and J. A. Sianoudis
6628_44 European Conference on Biomedical Optics (ECBO) 2007

Uncovering of melanin fluorescence in human skin tissue

Matthias Scholz, Goran Stankovic, Gunter Seewald, and Dieter Leupold
6633_73 European Conference on Biomedical Optics (ECBO) 2007

Multispectral autofluorescence diagnosis of non-melanoma cutaneous tumors

Ekaterina Borisova, Daniela Dogandjiiska, Irina Bliznakova, Latchezar Avramov, Elmira Pavlova, and Petranka Troyanova
7368_23 European Conference on Biomedical Optics (ECBO) 2009

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription