From research to routines – Ultrafast laser systems can be replaced by low cost lasers in two-photon excitation applications

Marko Tirri; Jonne Vaarno; Juhani T. Soini; Pekka Hänninen

doi:10.1364/ECBO.2003.5143_193

Novel Optical Instrumentation for Biomedical Applications
Proc. SPIE (Optica Publishing Group, 2003),
paper 5143_193
•https://doi.org/10.1364/ECBO.2003.5143_193

From research to routines – Ultrafast laser systems can be replaced by low cost lasers in two-photon excitation applications

Marko Tirri, Jonne Vaarno, Juhani T. Soini, and Pekka Hänninen

European Conference on Biomedical Optics 2003

Munich Germany
ISBN: 0-8194-5013-8

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Imaging Tools and Methods II (ITMII)

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M. Tirri, J. Vaarno, J. T. Soini, and P. Hänninen, "From research to routines – Ultrafast laser systems can be replaced by low cost lasers in two-photon excitation applications," in Novel Optical Instrumentation for Biomedical Applications, A. Boccara, ed., Vol. 5143 of Proc. SPIE (Optica Publishing Group, 2003), paper 5143_193.

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Abstract

This paper outlines the latest developments in two-photon excitation laboratory instrumentation using low-cost laser equipment. Our research group has shown that application of two-photon excitation need not to be limited to those laboratories that can afford the price and maintenance of ultrafast laser systems. With certain compromises and well-designed experiments, low-cost lasers, such as the Q-switched Nd:YAG microchip laser, can be used in utilizing the positive properties of two-photon excitation. Results using microparticles or cells as bioactive carriers confirm this fact. Application examples from biomedical research as well from clinical environment are presented.

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