Abstract

We developed a confocal microscope for transmitted light to visualize fine details in phase objects like unstained biological specimens. The main difficulty of confocal microscopy in transmitted light is the alignment of illumination and detector pinholes. This alignment was achieved by using „electronic pinholes“ on the detector side. In a first step, we were able to image cells in onion skin at greater depths, and with higher resolution, than by using conventional microscopy.

© 2003 SPIE