Fluorescence is widely used as a spectroscopic tool or for biomedical imaging. To extend these measurements to small concentrations or to fluorophores with very low quantum yield we have developed nanostructured substrates made of silver nanoparticles covered with a spacerlayer of alumina. Factors of about 200 are obtained for fluorescence enhancement with two photon excitation. Lifetime measurements reveal additional information on the decay channels induced by the nanoparticle presence.

© 2003 SPIE

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