Abstract

Fluorescent images that are blurred by large focus displacements are corrected by phase compensation. This is possible when the defocusing distance is obtained from an external measurement. A multi-modal digital holography that enables both quantitative phase imaging and 2D fluorescent imaging provides complimentary information from the same measuring object, such as living cells with fluorescent labels. In this paper, we adapted Zernike polynomials to correct the defocused fluorescent images, and used coefficients that are linear to the defocusing distances. Experimental results from bead images are presented.

© 2017 Optical Society of America