Abstract

We introduce and analyze a laser speckle micro-angiography (LSMA) to reconstruct the 2D visualization of the blood flows. The method takes the advantages of intensity fluctuation modulation (IFM), in which dynamic speckle pattern can be isolated effectively from stationary speckle pattern. Phantom flow experiments demonstrate that with IFM method the SNR and detected depth can be greatly improved. In vivo Biological experiments on the ears of rabbits and mice verify the outstanding performance of our LSMA in imaging microcirculatory network. this technique displays an importance potential application to develop a label-free optical micro-angiography in retinal imaging.

© 2015 Optical Society of America