Abstract

We propose a new strategy of three-dimensional (3D) tracking of living cells by digital holography microscopy based on the morphological changes of cells during the migration. We propose to use an approximation of the Tamura coefficient, as image contrast measure, for the estimation of the focal plane (i.e. the Z coordinate along the optical axis of the imaging systems) and a new morphological operator, from which we compute the transverse coordinates. A comparison with other 3D tracking methods is accomplished.

© 2013 Optical Society of America