Abstract

The use of multiple wavelength illumination in digital in-line holographic microscopy is presented. A biological sample is illuminated sequentially with three different optical sources and the separate holograms reconstructed with the appropriate wavelength. For controlling the scale of the reconstructed holograms, a reconstruction algorithm that allows for choosing the pixel size at the reconstruction plane independently of the wavelength and the reconstruction distance is used. The method is illustrated with experimental results.

© 2012 Optical Society of America

PDF Article
More Like This
Tracking of micrometer-sized objects with high-numerical aperture lensless digital holographic microscopy

John F. Restrepo and J. Garcia-Sucerquia
DSu3C.6 Digital Holography and Three-Dimensional Imaging (DH) 2012

Partially Coherent Digital In-line Holographic Microscopy

Jorge Garcia-Sucerquia, Diana Alvarez-Palacio, and Jurgen Kreuzer
DWB9 Digital Holography and Three-Dimensional Imaging (DH) 2009

In-line Digital Holographic Microscopy with Improved Phase Reconstruction

Anith Nelleri, Joby Joseph, and Kehar Singh
FWL5 Frontiers in Optics (FiO) 2007

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription