The use of multiple wavelength illumination in digital in-line holographic microscopy is presented. A biological sample is illuminated sequentially with three different optical sources and the separate holograms reconstructed with the appropriate wavelength. For controlling the scale of the reconstructed holograms, a reconstruction algorithm that allows for choosing the pixel size at the reconstruction plane independently of the wavelength and the reconstruction distance is used. The method is illustrated with experimental results.

© 2012 Optical Society of America

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