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Conferences > DH > 2010 > DTuB > DTuB7

Time-Domain Fluorescence Lifetime Optical Projection Tomography

J. McGinty, D. Stuckey, R. Laine, K.B. Tahir, M.A.A. Neil, J.V. Hajnal, A. Sardini, and P.M.W. French

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Abstract

We present a platform for measuring the fluorescence lifetime distribution in mesoscopic samples (~0.1–1cm) based on optical projection tomography and time-gated imaging. This is applied to optically cleared embryos expressing a calcium sensing FRET probe.

© 2010 Optical Society of America

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