Abstract
Using a blue light-emitting diode it is shown that Digital In-line Holography Microscopy (DIHM) with spherical waves can yield micron resolution even when large objects such as the head of a fruit fly are imaged. It is studied the influence of the size of the pinhole, thus varying the almost coherently illuminated area at the plane of the sample with the size of the object itself. Although resolution is less than that ultimately obtained with a fully coherent laser and a large numerical aperture, the use of a light emitting diode allows for a very compact and obviously low cost implementation of DIHM.
© 2009 Optical Society of America
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