Abstract

Dynamic, label-free imaging of cell metabolism using two photon excited fluorescence (TPEF) microscopy typically employs dual wavelength excitation of NADH and FAD+. The intrinsic fluorescence from these two cofactors can be used to calculate an optical redox ratio (ORR) that reflects the cellular metabolic state. In this work, we imaged MCF7 breast cancer cells under normal culture conditions and in response to the mitochondrial disruptors oligomycin and FCCP. By comparing TPEF with conventional oxygen flux analysis (seahorse assay) we validate the ORR for dynamic metabolic imaging over a range of relevant conditions.

© 2016 Optical Society of America