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Video-rate 3D Particle Tracking with Extended Depth-of-field in Thick Biological Samples

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Abstract

We present a single-aperture 3D particle localisation and tracking technique with a vastly increased depth-of-field without compromising optical resolution and throughput. Flow measurements in a FEP capillary and a zebrafish blood vessel are demonstrated experimentally.

© 2017 Optical Society of America

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