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Conferences > COSI > 2016 > CW5D > CW5D.6

Extended Field-of-view and Increased-signal 3D Holographic Illumination with Time-division Multiplexing

Samuel J. Yang, William E. Allen, Isaac Kauvar, Aaron S. Andalman, Noah P. Young, Christina K. Kim, James H. Marshel, Gordon Wetzstein, and Karl Deisseroth

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Abstract

We both extend the field-of-view and increase the signal in two-photon computer generated holography by using galvanometer mirrors to time-sequentially reposition multiple 3D holograms. We apply this toward 3D two-photon in vivo neuronal calcium imaging.

© 2016 Optical Society of America

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