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Conferences > CLEO_SI > 2015 > STh4K > STh4K.2

An Azimuthal Polarizer Assures Localization Accuracy in Single-Molecule Super-Resolution Fluorescence Microscopy

Matthew D. Lew and W. E. Moerner

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Abstract

Imaging only azimuthally-polarized light from single fluorescent molecules avoids emission from the z component of their transition dipole moments, resulting in accurate measurement of location regardless of emitter orientation and degree of objective lens misfocus.

© 2015 Optical Society of America

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