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Non-blinking "Giant" Nanocrystal Quantum Dots: Ideal Probes for Real-time Three-dimensional Tracking

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Abstract

We describe the development and characterization of two novel non-blinking “giant” nanocrystal quantum dot (g-NQD) systems and their application to single-particle tracking in live cells real-time and in three dimensions. The two g-NQDs – CdSe/CdS and InP/CdS – are characterized by a thick shell layer (>10 monolayers of CdS), a quasi-type-II or type-II electronic structure, and extremely suppressed non-radiative Auger recombination and photobleaching, in addition to suppressed blinking. We show that these unique traits make this new class of NQD ideal molecular probes for observing spatio-temporal dynamics of cellular processes.

© 2013 Optical Society of America

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