Directly studying the dynamics of nanoparticles with subwavelength dimensions, for example protein interactions or viral self-assembly, is difficult using conventional light microscopes due to Abbe’s resolution limit. Methods to overcome this limit such as fluorescence microscopy usually require to label particles and suffer from photobleaching in the case of long illumination times. A recent tracking method based on elastic light scattering from nano-objects inside a microstructured fiber which includes a nanometer sized channel managed to circumvent these limitations . However, due to the small channel size, this approach imposes high spatial constraints on the particle motion, impedes the investigation of multi-particle dynamics and allows very little control over the liquid flow inside the fiber.
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