Transmembrane proteins exhibit a wide variety of mobility in live cells, including diffusion in the plasma membrane and directed transport into the cell via endocytosis. Although a great deal has been uncovered about the biochemistry underlying these processes, their real-time visualisation in situ with the necessary nanoscopic resolution remains a daunting experimental challenge. Conventional microscopies rely on fluorescent labelling to monitor diffusion of such proteins within live cells. The resolution in both space and time by which one can successfully determine the position of the protein is curtailed by the finite and limited emission rate of the fluorophore.
© 2019 IEEEPDF Article
More Like This
André Gemeinhardt, Katharina König, Francesca Nicoli, Mahyar Dahmardeh, and Vahid Sandoghdar
jsii_2_3 European Quantum Electronics Conference (EQEC) 2019
Michelle Küppers, Anna Kashkanova, Jennifer Lühr, David Albrecht, Alexandra Schambony, and Vahid Sandoghdar
MW1A.4 Microscopy Histopathology and Analytics (Microscopy) 2020
STh3B.2 Optical Sensors (Sensors) 2012