Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • 2017 European Conference on Lasers and Electro-Optics and European Quantum Electronics Conference
  • (Optica Publishing Group, 2017),
  • paper CL_P_2

Optical Electrophysiology: Femtosecond Laser Facilitated Electrophysiological Measurements from Single Cells

Not Accessible

Your library or personal account may give you access

Abstract

One of the indispensable tools of electrophysiology is patch-clamp recording, which enables direct observation of ionic transport through cellular membranes. It is an invaluable research tool, providing experimental information related to cellular electrical metabolism, as well as various pathological conditions. While the inventors of patch-clamp recording subsequently became Nobel laurates [1], still it can be considered as one of the most complicated experimental setups, due to the difficulty in puncturing cells using capillary microelectrodes with extreme precision. The throughput of the measurement is also considerably low. Thus, automatized planar patch-clamp systems depending on automatic suction of cells using special chips have emerged, considerably increasing the throughput of electrophysiological measurements for about a decade [2]. However, they are generally restricted to be used only with cultured cells, while they have a poor applicability when isolated cells are considered. Real-time observation of the cells for quality assessment, in terms of the measurement, is also not possible.

© 2017 IEEE

PDF Article
More Like This
Optical and Hydrodynamic Stretching of Single Cells from Blood

Henrik Thirstrup, Tony B. Rungling, Mustafa Zyad Khalil Al-Hamdani, Ragavan Pathanchalinathan, Morten Hanefeld Dziegiel, Anders Kristensen, Rodolphe Marie, and Kirstine Berg-Sørensen
OtM4E.1 Optical Trapping Applications (OMA) 2017

An ultra-widefield microscope for high-speed, all-optical electrophysiology

Christopher A. Werley, Steven F. Nagle, John M. Ferrante, and Steven C. Wasserman
BrM4B.3 Optics and the Brain (BRAIN) 2017

Optical Manipulation of Single Cells in Femtosecond Laser Fabricated Lab-on-chip

R. Martinez Vazquez, F. Bragheri, P. Minzioni, N. Bellini, P. Paiè, G. Nava, R. Ramponi, I. Cristiani, and R. Osellame
CL_6_5 The European Conference on Lasers and Electro-Optics (CLEO/Europe) 2013

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved