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  • 2017 European Conference on Lasers and Electro-Optics and European Quantum Electronics Conference
  • (Optica Publishing Group, 2017),
  • paper CL_4_6

Detecting Metabolic Carbon Dioxide Using a Tunable Laser for Non-Invasive Monitoring Of Growth of Bacterial Pathogens

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Abstract

The emergence of antibiotic resistance of bacteria to many major antibiotics has made the study of the growth of bacterial pathogens very important in the discovery and development of novel drugs [1, 2]. Metabolic activities in bacterial cultures lead to concomitant emission of a variety of gases. Accurate measurement of the mole fractions of these gases is an indirect but reliable measure of bacterial growth. Traditional techniques that are used to quantify these gases that accumulate in the headspace of the bacterial culture vessels have several drawbacks. They are either destructive, or slow, or do not work in real-time or are invasive. Tunable diode laser spectroscopy (TDLS) is a technique that makes it possible to accurately measure the growth of the bacterial pathogens non-invasively and non-destructively. It is possible to avoid the intermediate steps of sample extraction and preparation that are the major reasons for contamination. This ensures that the growth process of the bacterial cultures proceeds undisturbed. Additionally, real time monitoring makes it possible to rapidly assess the efficacy of newly developed anti-microbial drugs. There is evidence to suggest that such rapid assessment reduces mortality rates and cuts hospitalization costs [2].

© 2017 IEEE

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