Abstract

Digital holographic microscopy using only a CCD camera and a simply in-line lensless optical setup has been demonstrated to be a novel tool to obtain 3D images of static or moving objects with micrometer resolution. In this technique the light scattered from the object/sample interferes with the reference incident light to generate a holographic pattern at the camera plane. Then, the recorded intensity is numerically processed, and the object wavefront is reconstructed. Applications of digital lensless holography microscopy (DLHM) include the investigation of in situ organisms and their motion in plankton [1], researching on microbial life forms in the Canadian High Arctic [2], tracking micrometer sized particles with high NA [3], or analyzing transparent phase objects under femtosecond illumination [4].

© 2015 IEEE