Abstract

Reversible permeabilization of live cells is a complex and increasingly addressed issue, whether it is for medical application, or in lab research protocols where a constant necessary effort is made to reach more realistic investigation conditions in biological system. It is characterized by increased molecule transfers through the cell membrane. Applications cover anticancer drugs or imaging markers deliveries, gene therapy… Besides complex viral vectors that rise potential immune issues, delivery of biomolecules can be done mechanically or chemically. Reversible permeabilization in mostly obtained by techniques creating pores into the membrane, the most commons being electroporation, use of non-ionic detergent and pore-forming toxins [1-2].

© 2015 IEEE