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Conferences > CLEO_Europe > 2013 > CL_3 > CL_3_2
  • 2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference
  • (Optica Publishing Group, 2013),
  • paper CL_3_2

Quantifying molecular colocalization in live cell fluorescence microscopy

Fabian Humpert, Idir Yahiatène, Martina Lummer, Markus Sauer, and Thomas Huser

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Abstract

One of the most challenging tasks in microscopy is the quantitative identification and characterization of molecular interactions. In living cells this task is typically performed by fluorescent labeling of the interaction partners with spectrally distinct fluorophores and imaging in different color channels. Potential interaction can then be determined by parameters that can measure the degree of molecular colocalization.

© 2013 IEEE

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