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  • 2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference
  • (Optica Publishing Group, 2013),
  • paper CD_4_4

Scanless two-photon microscopy with a 30 fs laser by means of a diffractive dispersion compensation module

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Abstract

Scanless two-photon microscopy uses a spatial light modulator (SLM) to shape the incoming laser beam into any user-defined light pattern. These microscopes, that do not contain mechanically moving parts, improve the temporal resolution of conventional scanning multiphoton microscopy since they highly mitigate the severe temporal limitations inherent to sequential scanning of the sample [1]. However, diffractive optical elements encoded into a SLM for multiphoton microscopy prevent the use of ultrafast sources (say, pulses shorter than 100 fs) due to huge dispersion (both spatial and temporal), which limits high resolution nonlinear excitation [2]. We have engineered a simple dispersion-compensated module (DCM) based on the diffractive lens-pair configuration shown in Ref. [3] that permits to extend the range of pulsed sources available for scanless multiphoton microscopes to ultrashort femtosecond pulses. In this way, we strongly alleviate some unwanted effects (specifically, spatial chirp and pulse-front tilt).

© 2013 IEEE

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