Abstract
Coherent anti-Stokes Raman scattering (CARS) is a nonlinear Raman process in which pulsed light of at least two frequencies is mixed within a sample. By overlapping pump light at a frequency of ωp and Stokes light at ωs temporally and spatially, CARS light at a frequency of 2ωp-ωs is produced. When the frequency difference (ωp-ωs) can be tuned to specific vibrational resonances within a sample, CARS microscopy provides intrinsic vibrational contrast [1]. However, in the case of the use of ultrabroadband pulses as an excitation light source, the resonant CARS signal is accompanied by significant nonresonant CARS signals from the electronic contribution. Fourier-transform CARS (FT-CARS) spectroscopy provides the suppression of the nonresonant CARS signals and the measurement of broadband CARS spectra with high spectral resolution at a time [2]. In this paper, we demonstrate that FT-CARS microscopy employing 5-fs ultrabroadband pulses can be applied to imaging of unstained HeLa cells.
© 2009 IEEE
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