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Through Cuticle Neural Imaging of Drosophila Melanogaster with Multiphoton Microscopes

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Abstract

Multiphoton neural imaging of Drosophila melanogaster required the removal of dorsal head capsule cuticle, prohibiting longitudinal imaging studies (> 5hrs). Here we demonstrate two- and three-photon imaging on cuticle-intact Drosophila brains with dorsal air-sacs removed.

© 2020 The Author(s)

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