Here we have developed a novel large-field high-resolution two-photon digital scanned light-sheet microscopy (2P3A-DSLM) based on ultrafast axial scanning of illumination focal spot with a tunable acoustic gradient (TAG) index device. This new technique provides a sub-micron axial resolution with a large field of view in deep tissues. In vivo imaging with 2P3A-DSLM resolves functional maturation of pancreatic beta cells and neuron circuit activities in live Zebrafish embryos.

© 2016 Optical Society of America

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