Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Light Paves the Way to Single-Molecule Detection and Photocontrol, Foundations of Super-Resolution Microscopy

Open Access Open Access

Abstract

More than 25 years ago, low temperature experiments aimed at establishing the ultimate limits to optical storage in solids led to the first optical detection and spectroscopy of a single molecule in the condensed phase. At this unexplored ultimate limit, many surprises occurred where single molecules showed both spontaneous changes (blinking) and light-driven control of emission, properties that were also observed in 1997 at room temperature with single green fluorescent protein variants. These observations form foundations for super-resolution microscopy beyond the diffraction limit with single molecules, and tracking of single molecules in cells continues to yield surprises.

© 2015 Optical Society of America

PDF Article
More Like This
Light Paves the Way to Single-Molecule Detection and Photocontrol, Foundations of Super-Resolution Microscopy

W. E. Moerner
JW1A.1 Adaptive Optics: Analysis, Methods & Systems (AO) 2015

Common fluorescent proteins for single-molecule localization microscopy

Natalia V. Klementieva, Nina G. Bozhanova, Natalie M. Mishina, Elena V. Zagaynova, Konstantin A. Lukyanov, and Alexander S. Mishin
953609 European Conference on Biomedical Optics (ECBO) 2015

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved